(Cytoming Cytometry, FCM) mai ƙididdigar tantanin halitta ne ya auna ƙarfin tsananin alamomin sel. Fasahar fasaha ce ta samo asali ne bisa bincike da rarraba sel guda. Zai iya auna sauri da rarrabe girman, tsarin ciki, DNA, RNA, sunadarai, antigens da sauran kwayoyin halitta, kuma ana iya kasancewa bisa tarin ƙwayoyin sel.
Gudun Cytometer yana kunshe da waɗannan sassa guda biyar:
1 Ciki mai gudana da kayan ruwa
2 Laser Haske da Tsarin Daske Tsarin
3 tsarin naptical
4 Wutar lantarki, adanawa, nunawa da tsarin bincike
5 tsarin rarraba kwayar halitta
Daga gare su, lerning lernight a cikin laseran tushen laser da tsarin ƙirar shine babban ma'aunin siginar kyalli a cikin Cytometcence. Ifin girman hasken numfashi da kuma lokacin murmurewa yana da alaƙa da tsananin sigina sigina. Laser ne mai haske mai haske wanda zai iya samar da guda-covengthing, babban-tsananin, da kuma zafin shakatawa mai haske. Tushen hasken wutar lantarki ne mai kyau don biyan waɗannan buƙatun.
Akwai ruwan tabarau guda biyu tsakanin laker asalin da kuma ɗakin kwarara. Wadannan ruwan tabarau sun mayar da hankali da laseran laser tare da sashe-tsini-sashen da aka kawo daga katako mai tushe tare da ƙaramin giciye-sashe (22 μm × 66 μm). Kogin Laser a cikin wannan katako na elliptical yana rarraba gwargwadon rarraba al'ada, tabbatar da ingantaccen haske mai zurfi ga sel gano yankin Laser. A gefe guda, tsarin gani kuma ya ƙunshi lakunan ruwan tabarau da yawa na ruwan tabarau, pin plholes, da kuma matattara, wanda za'a iya raba shi zuwa ƙungiyoyi biyu: sama da kuma za a iya raba shi zuwa ƙungiyoyi biyu: sama da kuma za a iya raba shi zuwa ƙungiyoyi biyu: sama da kuma za a iya raba shi zuwa rukuni biyu.
Tsarin gani a gaban Chilber Resenter ya kunshi ruwan tabarau da Pinhole. Babban aikin ruwan tabarau da Pinhole (galibi ruwan tabarau biyu da kuma Pinhole) shine don mai da hankali da layin laser tare da ƙaramin yanki tare da ƙananan giciye-sashe. Wannan yana rarraba makamashi na laser bisa ga rarraba al'ada, da tabbatar da haske mai haske ga sel a fadin yankin da aka gano Laser.
Akwai manyan nau'ikan matattarar guda uku:
1: Long Pass Tace (LPF) - yana ba da haske kawai tare da igiyar ruwa sama da takamaiman darajar don wucewa.
2: Short-Pass tace (SPF) - kawai yana ba da haske tare da igiyar ruwa da ke ƙasa takamaiman darajar don wucewa.
3: Bandara tace (BPF) - yana ba da haske a cikin takamaiman kewayon ƙaura don wucewa ta.
Hanyoyi daban-daban na masu tacewa na iya kai tsaye alamomi masu kyalli a cikin igiyar ruwa daban-daban ga mutum bututun mai daukar hoto (pmts). Misali, matattara don gano launin kore mai kyalli (Fitc) a gaban PMT sune LPF550 da BPF5255. Filesan da aka yi amfani da su don gano launin orange-ja a gaban PMT sune LPF600 da BPF575. Mace don gano launin ja (Cy5) a gaban PMT sune LPF650 da BPF675.
Ana amfani da Cytometry na gudana don rarrabawa tantanin halitta. Tare da ci gaban fasaha na kwamfuta, haɓakar ƙwayar ƙwaƙwalwar rigakafi da kuma ƙirƙirar fasahar monochlonal ta antoclonal, aikace-aikacen sa a ilmin halitta, magunguna, kantin magani da sauran filayen suna kara yadu sosai. Waɗannan aikace-aikacen sun haɗa da nazarin tantanin halitta, Apoptosis na sel na ƙwayar cuta, ƙwayar cuta, da sauransu.
Lokacin Post: Satumba 21-2023
